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Taylor-Wharton Freezing Tray User Manual

Handi-freeze freezing tray, Operation, Operating tips

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For 35HC, 35VHC, 36VHC

or 750RS Refrigerators

TW-51

HANDI-FREEZE

FREEZING TRAY

The Handi-Freeze tray provides an economical method of

freezing biologicals when a 35HC, 35VHC and 36VHC or

750RS Cryogenic Refrigerator is available. This acces-

sory replaces the necktube core of the four refrigerator

modules and allows the used to take advantage of the

cold nitrogen gas in the necktube for freezing purposes.

When materials is placed in the tray, the depth of the tray

may be adjusted by turning the knob on the top of the

unit. Clockwise rotation will move the tray upward toward

the warmer portion of the necktube; counter clockwise

rotation will move the tray deeper into the colder area.

SAFETY NOTICE – Do not attempt to use this accessory

until you read and understand the safety precautions for

cryogenic refrigerators that are found in Form TW-10

“Handle with Care”. The improper handling of cryogenic

fluids can cause personal injury.

Operation

The tray is designed to hold 24 vials, 12.5 mm in diameter or

less. The freezing rates involve many variables and must be

determined by the user from actual test data. In general, the

top tray position is used for temperature down to 0°F (-

18°C), the middle position to -50°F (-46°C), and the lower

position to cryogenic temperatures -200°F (-129°C).

Convenient index numbers on the freeze tray guide rods aid

in indexing freeze rates. The tray positioning control will

change the tray location by approximately ¼ in. (6.4 mm) for

each complete turn of the knob.

The necktube core of the refrigerator (35HC, 35VHC,

36VHC or 750RS) must be removed and the Handi-Freeze

tray can be inserted into the necktube. The canisters/racks,

if used, need not be removed, as the freeze tray design has

allowed clearance for the canister/rack handles.

Operating Tips

§

Frost formation may make operation of the mecha-

nism difficult. If the cold unit is exposed to moist air,

frost will form. If frost formation, makes the control

knob difficult to turn, remove the unit, and allow it to

warm-up, then dry the moisture with warm air or a dry

cloth.

§

Remove the Handi-Freeze tray from the refrigerator

when not in use. The unit is not insulated and its use

as a replacement for the insulated necktube core

provided with the refrigerator will result in much

greater liquid nitrogen consumption.

§

When removing the Handi-Freeze tray, use the

indentations on the cap. Do not use the tray position

control know as a handle or the unit may be dam-

aged.

§

The refrigerator should be at least half full of liquid

nitrogen.

§

A cooldown time of a least one day should be al-

lowed after filling a warm refrigerator. Allow one hour

after adding liquid refrigerant or after the addition of a

warm specimen canister/rack.