Dako Hybridizer - Manual (Page 2)

PGM: Program
Denat & Hyb: Denaturation & Hybridization
Denat Temp: Denaturation Temperature
Denat Time: Denaturation Time
Hyb Temp: Hybridization Temperature
Hyb Time: Hybridization Time
Hyb Only: Hybridization Only
Fixed Temp: Fixed Temperature
Heating to Fxd: Heating to Fixed (-- °C)
Display Abbreviations
Humidity Control Strips
Slide Location
Select Program Type
Abort Program in Process
n Minimize evaporation of sample from prepared slides.
n To install strips, lift lid and insert strips into slot positions.
n Saturate strips with distilled water or equivalent.
n Strips should be replaced frequently as they will deteriorate
over time and with use.
n Accommodates up to 12 slides per run.
n The Hybridizer prompts to add slides.
n Lift lid and load slides onto plate using slide locator for
easy positioning.
n Frosted and/or label end of slides should be away from
slide locator.
n Move slides across plate until they rest against slide locator.
Note: If slides are not resting against slide locator, closing of lid may
break slides.
n The Hybridizer is capable of storing 40 programs. Each
program can be one of three program types:
- Denaturation and Hybridization
- Hybridization Only
- Fixed Temperature
n Select program type and follow screen prompts to enter
run times and set temperatures.
n Press “STOP” button to abort a program in process.
n Use arrows to move cursor to “Yes” line.
n Press “Enter” button to accept.
S e l e c t P G M Ty p e
D e n a t & H y b
H y b O n l y
F i x e d Te m p
A B O R T I N G ! !
A r e Yo u S u r e ?
N o
Ye s - M a i n M e n u