SciGene Hybex Microarray Incubation System - Manual (Page 13)

Hybex® Microarray Incubation System — USER MANUAL
Version 1.1E, August 2012 12 (408) 733-7337, [email protected]
VIII. APPENDIX A – Using and Calibrating a Watlow SD31 Controller
For selected Hybex® incubators manufactured between 2005 and 2012.
A. Using the Watlow SD31 Temperature Controller
The SD31 controller has a single LED panel with four buttons. The set
temperature is displayed if the SET key is pressed and held otherwise the
ACTUAL block temperature is displayed.
To set the block temperature:
1. Insert a block into the instrument and power on.
2. Hold in the SET button to view the previous set temperature.
3. While holding down the SET button, press the up or down arrow button until the
desired set temperature is shown.
4. Release the SET button to display the actual temperature. The instrument will adjust
block heating until the SET temperature is attained.
B. Calibrating the Watlow SD31 Temperature Controller
Re-calibrating the SD31 controller is recommended only if the display varies by more than
one degree (1°C) to a connected NIST-certified digital thermometer (SciGene cat. #1051-52-0).
To adjust the controller to achieve accurate temperatures:
1. Insert a block into the Hybex® incubator, turn ON the instrument and set the
controller to 65°C. Allow 15 minutes to stabilize.
2. Using the cable provided with the digital thermometer, plug one end into the blue
receptacle found on the back panel of the Hybex® incubator and the other end into
the digital thermometer.
3. Turn on the thermometer. Allow 1 minute for thermometer to stabilize. The actual
temperature of the block will be displayed.
4. Calculate the difference in the temperature shown on the controller and the digital
thermometer. For example, if the controller displays 65.0°C and the digital
thermometer displays 66.5°C, the difference (offset) is 1.5°C.
5. On the temperature controller, press the Infinity Key (∞) for
three seconds until “OPEr” appears.
6. Press the down arrow four times until “Cal” appears.
7. Press and hold the SET key. The existing offset value between
the controller and digital thermometer is displayed.
8. Press and hold the SET key and use the up/down arrows to
adjust the offset value to the temperature difference calculated in Step 4. For
example, if the controller shows a temperature 1.5°C lower than the thermometer,
adjust the offset by adding 1.5 to the value shown.
9. Press the Infinity Key (∞) to exit calibration and return to the operation display.
Verify that the temperature on the thermometer matches the display. Your
incubator is now calibrated to provide accurate temperatures between 35 and 99°C.