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Environmental Express Smart Bottle User Manual

Page 6

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Environmental  Express,  Inc.                                                                                Smart  Bottle  Rack  Operation  and  Instruction  Manual    

 

         800.343.5319/843.881.6560  •  www.envexp.com  

 
 

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All connections should be hand tight only. Over tightening these connections could cause them to
weaken or crack and compromise the integrity of the seal.

Using the labels attached to the tubing as a guide; attach the other end of the reagent umbilical cord
to the back of the SPE-Express. The connections are the same style as were connected to the Smart
Bottle Rack. All connections should be hand tight only. Over tightening these connections could cause
them to weaken or crack and compromise the integrity of the seal.

Attach the quick connect of the gas pressure from the SPE-Express and the vacuum source to the
appropriately labeled barb on the side of the Smart Bottle Rack.
NOTE – Do not connect the gas pressure directly from the supply to the Smart Bottle Rack. Doing so will
result in unsafe pressure levels within the system and bottles. The SPE-Express has a pressure regulator
that will step the pressure down to safe levels.

Connect the 25-pin cord to the Smart Bottle Rack and the SPE-Express.

Turn on power, vacuum, and gas pressure and log in to the SPE-Express software.

2.2.2. Converting from the default bottle harness

Remove the Reagents Pressure Hose from the SPE-Express unit. (This will allow the SPE-Express
components to retain pressure while the bottles and bottle harness are depressurized).

Loosen the caps on the n-Hexane, Methanol, and DI Water. DO NOT REMOVE CAPS FROM BOTTLES.

Ensure there is a filter cup assembly in place on the swinging assembly and that the swinging assembly
is in the UP (LATCHED) POSITION.

Ensure the lower glass chamber is seated on the upper glass chamber and secured with the glass
clamp.

Enter the Station 1 tab of Manual Screen in the SPE-Express software.

Click to open the “Hexane Waste Sol”.

Ensure that all n-Hexane waste has entered the Hexane waste container and click to close the “Hexane
Waste Sol”.

Click to open the “Water Miscible Waste Sol”.

Ensure that all water miscible waste has entered the water miscible waste container and click to close
the “Water Miscible Waste Sol”.

Click the “Hexane Chamber Rinse Sol” to drain the hexane from the chamber rinse line back to the n-
Hexane source bottle.

Click the “Nitrogen Pressure Sol” button.

Once the n-Hexane is evacuated from the line (Nitrogen will be heard entering the n-Hexane source
bottle), close the Hexane solenoid by pressing “Hexane Chamber Rinse Sol”.

Click to open the “Eluted Sample Path Sol”.

Click to open the “Hexane Bottle Rinse Sol”.

Once the n-Hexane is evacuated from the line (Nitrogen will be heard entering the n-Hexane source
bottle), close the Hexane solenoid by pressing “Hexane Bottle Rinse Sol”.

Click to open the “Methanol Rinse Sol”

Once the Methanol is evacuated from the line (Nitrogen will be heard entering the Methanol source
bottle), close the Methanol solenoid by pressing “Methanol Rinse Sol”.

Click to open the “DI Water Rinse Sol”

Once the DI Water is evacuated from the line (Nitrogen will be heard entering the DI Water source
bottle), close the DI Water solenoid by pressing “DI Water Rinse Sol”.