5 removing the gel, 6 maximum well/comb volumes – C.B.S. Scientific DASG-250-02 User Manual
Page 14
3.5
Removing the Gel
1. Turn the power supply off and disconnect the leads from the power supply. Remove the
safety cover from the unit, by placing thumbs on white posts next to red & black connectors,
then pushing down while pulling up with fingers under lid. DO NOT pull on power cords.
2. Drain upper reservoir using a pipette or syringe. Detach side clamps and remove gel
sandwich. Carefully separate, making sure that the gel remains attached to one plate. Stain
and fix according to your preferred method. Dispose of buffer according to your institutions
safety requirements.
3.6 Maximum Well/Comb Volumes
NOTE: To calculate sample well volume expressed in millimeters (mm) of height divide
maximum volume by tooth depth.
TABLE 1---Combs
Overall
Length
13cm
Tooth
width/
mm
Tooth
Depth
Spacing
between
teeth
mm
0.75mm
thickness
volume per
tooth
microliters
(ul)
1.0mm
thickness
volume per
tooth
microliters
(ul)
1.5mm
thickness
volume per
tooth
microliters
(ul)
2.0mm
thickness
volume per
tooth
microliters
(ul)
3.0mm
thickness
volume per
tooth
microliters
(ul)
1
130.2
19.1
3.2
1865
2486
3729
4966
7458
2
63.5
19.1
3.2
909
1213
1819
2426
3639
3 equal
41.3
19.1
3.2
591
788
1182
1576
2364
2 w/middle
marker
59.8/
4.2
19.1
3.2
856
1142
1713
2284
3426
4
30.1
19.1
3.2
432
576
1151
1152
1728
5
23.5
19.1
3.2
336
449
673
898
1347
6
19.1
19.1
3.2
273
364
546
728
1092
8
13.5
19.1
3.2
193
257
386
514
771
10
10.1
19.1
3.2
145
194
291
388
582
12
7.9
19.1
3.2
113
151
227
302
453
14
6.4
19.1
3.2
91
121
182
242
363
15
5.7
19.1
3.2
82
109
163
218
327
16
5.2
19.1
3.2
74
98
147
196
294
20
4.2
17.7
2.4
55.72
74.3
111.45
148.7
222.9
29
2.9
17.7
1.6
38.5
51.33
77
102.66
154.0
30
2.8
17.7
1.6
37.2
49.6
74.4
99.12
148.8