C.B.S. Scientific DASG-250-02 - Manual (Page 12)

www.cbsscientific.com 12 Adjustable Slab Gel Manual 07/13
5. Lift the assembly and stand it on the base of the
clamps. For leveling, push glass plate assembly
down until it stops against clamp body. Clamp
the sides of the assembly with additional casting
clamps on either side. As each clamp is
attached, be sure the gasket is aligned between
the plates forming a seal.
6. Apply PAGE or agarose solution to gel plate sandwich using a syringe or pipette. If
using a stacking gel, pour desired height of running gel, then overlay a small amount
of dH
2
O or 0.1% SDS solution to top of gel. After polymerization, rinse with buffer,
add stacking gel solution and insert comb. For regular, unit percentage gels, add
polyacrylamide solution to correct height, and insert comb. Allow gel to set, usually
20 minutes. Extra gel solution in pipette or syringe can be monitored to test
polymerization of gel mix.
7. Disassembly. Hold the clamped plate
assembly with one hand. Remove the gasket
by starting at one of the top ends and pulling
up and out on the gasket until it releases from
the plate, up to the bottom of each of the
white clamps. When each clamp is reached
DO NOT remove it, instead feed the gasket
down through the clamp body and repeat
pulling up and out. Continue feeding until the
gasket is fully detached. If gel, is not to be
used immediately, wrap entire plate sandwich
with plastic wrap tightly to seal and store at
4°C for up to a month.