C.B.S. Scientific DSG Series - Manual (Page 8)

C. B. S. œ Scientific 8 Dual Slab Gels
SECTION 3
Instructions for Use
3.1
Dual Slab Gel Unit Preparation
1. Place Dual Slab Gel unit on a level work surface in an authorized work area. Level the unit
by adjusting the four white nylon thumbscrew until the bubble is centered in the level. Be
sure that all four of the screws are touching the bench.
2. Verify that the comb, spacer set and Gel Wrap
™ gasket are the same thickness, by
assembling without gel casting.
3. To use the internal cooling option, attach tubing to the inlet (bottom port) and outlet coolant
ports (top port) and to your pump.
3.2 Preparation/Cleaning of Glass Plates
Hand wash both plates with a high quality lab detergent followed by a complete rinsing with
dH
2
O. Air dry or use a lint-free tissue. Spray/wipe the chosen inner surfaces of the plate set with
95% ethanol and dry with lint-free tissue.
3.3 Gel Casting Using Gel Wrap
™ Gasket Casting method
1. Start by holding the rectangular back plate with
the rounded bottom corners and start applying
the gasket around one side of the glass plate.
Note: one side of the “U” shaped gasket is flat,
and the other side has tubing that will act as a
seal around the spacers.
2. When applying the gasket over the rounded
corners of the back glass plate, make sure the
notches on the gasket align with the rounded
corners of the glass plate. Once the gasket is
pushed over the bottom edge and corners, work
it down the remaining side.
3. Place the gasketed plate on the lab bench with
the tubing side up, and extend the bottom of the
plate over the edge of the bench, approximately
¾ of an inch. Place the spacers along side the
inside edges of the gasket. Be sure the rounded
corner end of each spacer is facing the outside
bottom of the plate, following the radius of the
glass.