C.B.S. Scientific DSG Series - Manual (Page 10)

C. B. S. œ Scientific 10 Dual Slab Gels
3.4 Running the Gel
1. Attach plate set(s) to unit with smaller
or notched plate facing towards the
upper buffer reservoir. If using a pre-
cast gel stored at 4
°C, allow to warm
to room temperature. Use white
clamps with mouth open (cat. # GPC-
0001) to attach plate set to unit,
clamping each side to the sandwich.
2. Pour freshly prepared buffer in upper
and lower chamber. Using a pipette
or syringe, thoroughly flush out the
wells in the glass plate sandwich with
buffer from the upper chamber. Boil
or heat samples and then place
immediately on ice. Load samples. If
outer lanes do not contain sample, it
is recommended that you run
standards (Rainbow™ standards can
be monitored easily during run)
and/or fill outer lanes with loading
buffer to reduce smiling and wrap-
around effects.
3. Attach safety cover.
4. Connect the leads to the power
supply matching the color-coded
red to red and black to black. See
Section 4.1 for recommended
power conditions.
Begin separation by electrophoresis.