C.B.S. Scientific ECU-040 - Manual (Page 5)

C.B.S. Scientific Electro-Eluter Concentrator 5
SECTION 1
General Information
1.1 Introduction
The Electro-Eluter/Concentrator is designed to elute and concentrate macromolecules
simultaneously from acrylamide or agarose gels. Typical applications include recovery of
proteins from SDS-acrylamide gels, and of restriction enzyme-treated DNA from agarose gels.
The elution of proteins from gel strips has become a useful technique for preparing proteins for
primary structure analysis because this procedure can be used after Coomassie staining to
visualize the location of the protein bands in the gels.
Eluter/Concentrator blocks consist of a precision-machined sample recovery channel and two
silicone-Teflon® washer/open-top cap assemblies for holding dialysis membranes securely in
place.
The three-chamber Electro-Eluter is designed to allow buffer cycling. The buffer drain outlets on
the side for the two electrode chambers allow overflow buffer to drop into the mixing chamber.
The mixed buffer in then pumped back into the electrode chambers. This prevents build-up of a
pH gradient, which can occur during long-term electrophoresis, interfering with the ionic migration
of the protein or DNA sample. The buffer cycling system also eliminates the need to change
buffer during electrophoresis.
1.2 Specifications
Constructions
Buffer chamber, lid Acrylic, mirror
Electrodes Platinum wire .012" diameter
Power cords FR Urethane, rated 7500V,
200mA, 65° C
Blocks Acrylic
Caps Bakelite*
Washers TFE/Silicone
Safety Certification EN61010-1-1993 (IEC1010-1)
ECU-040 ECU-040-25 ECU-040-20 ECU-040-50 ECB-102 ECB-105
Shipping weight 7lbs 8lbs 8lbs 8lbs 2lbs 2lbs
Overall Size
(l)x(w)x(h) cm
30.8x20.3x12.
7
30.8x20.3x12.
7
30.8x20.3x12.7 30.8x20.3x12.
7
1.9x5.7x2.54 5.7x5.7x2.54
Recommended
buffer volume: 550 550 550 550 - -
Loading chamber
capacity ___ ___ ___ __ 2.0ml 5.0ml
Migration channel
volume ___ ___ ___ ___ 5.0ml 12.0ml
Sample recovery
volume ___ ___ ___ ___ 200ul 500ul
Distance between
electrodes 15cm 15cm 15cm 15cm ___ ___
Voltage limit 250 250 250 250 ___ ___