5 maximum well/comb volumes, Table 1---combs for 14cm wide unit, Table 2---combs for 20cm wide unit – C.B.S. Scientific GCSGE-040-02 User Manual
Page 11
C.B.S. Scientific
Econo-Sub Productivity Package
11
3. Gently lift the gel tray from the unit. Always wear gloves, eye protection and protective
clothing if buffer and/or gel contains Ethidium Bromide. Ethidium Bromide is a powerful
mutagen, gloves, eye protection and protective clothing should always be worn when
handling the gel or buffer solutions. See Material Data Safety Sheets.
4. View separated fragments under UV light, using proper protection for eyes and skin (see
manufacturer’s instructions).
3.5 Maximum Well/Comb Volumes
NOTE: To calculate sample well volume expressed in millimeters (mm) of height, divide maximum
volume by tooth depth.
TABLE 1---Combs for 14cm wide unit
SGC14
Combs for
14cm wide
unit
# of wells
Tooth
Depth
mm
Spacing
between
teeth
mm
Overall
length
of comb
mm
Tooth
width/
mm
1.0mm
thickness
volume per
tooth
microliters
(ul)
1.5mm
thickness
volume
per tooth
microliter
s (ul)
2.0mm
thickness
volume per
tooth
microliters
(ul)
3.0mm
thickness
volume per
tooth
microliters
(ul)
1
12.7
3
120.64
120
1524
2286
3048
4572
3E
12.7
3
120.64
38.1
483.87
725.81
967.74
1451.61
5
12.7
3
120.64
21.6
274
411.48
548
822
10
12.7
3
120.64
9.2
117
175.26
232
350
12
12.7
2.4
120.64
7.8
99
148.59
198
297
13
12.7
2.4
120.64
6.6
83.82
125.73
167.64
251.46
16
12.7
2.4
120.64
5.3
67.3
100.9
134.6
201
20
12.7
1.6
120.64
4.5
57
85.7
114
171
26
12.7
1.6
120.64
3.1
39.3
59
78
118
27
12.7
1.6
120.64
2.93
37.21
55.82
74.42
111.63
TABLE 2---Combs for 20cm wide unit
SGC20
Combs for
20cm wide
unit
# of wells
Tooth
Depth
mm
Spacing
between
teeth
mm
Overall
length
of comb
mm
Tooth
width/
mm
1.0mm
thickness
volume per
tooth
microliters
(ul)
1.5mm
thickness
volume
per tooth
microliters
(ul)
2.0mm
thickness
volume per
tooth
microliters
(ul)
3.0mm
thickness
volume per
tooth
microliters
(ul)
1
12.7
3.17
180
180
2286
3429
4572
6858
3E
12.7
3.17
180
49.73
631.57
947.35
1263.14
1894.71
3M
12.7
3.17
180
73.66
935.48
1403.22
1870.96
2806.44
5
12.7
3.17
180
33
419
628
838
1257
8
12.7
2.38
180
20.5
160.3
390
520.6
780
12
12.7
2.38
180
12.8
162.5
243
325
487.6
16
12.7
2.38
180
9
115
172
230
345
20
12.7
2.38
180
6.7
86.1
129
172
258
30
12.7
1.57
180
4.5
57
85.5
114
171
40
12.7
1.57
180
3
37.97
56
75
113