C.B.S. Scientific GCMGU-202T - Manual (Page 10)

10
Horizontal Mini-Gel Instructions 9-12-13
3.4 Running the Gel
1. Add enough running buffer to fill both
reservoirs and overflow the surface of the
gel to a depth of 2-3mm. Flush out any air
bubbles in the wells. Stains may be added
during this step. Use according to manufac-
turer’s recommendations.
2. Load the samples into sample wells. Do not
forget to load DNA size standard ladders.
For best visibility load samples on contrast-
ing background color such as red or black.
Safe stains may be added during this step.
Use according to manufacturer’s recom-
mendations.
3. Place the safety cover on the unit. Connect
power leads to power supply, matching the
color-coded red to red and black to black.
See Section 4.1 for recommended power
conditions. Begin separation by electro-
phoresis.
3.5 Removing the Gel
1. Turn power supply off and disconnect
the leads from the power supply. Remove
the safety cover from the Mini-Gel unit by
placing thumbs on white posts and pushing
down while pulling up with fingers under lid.
DO NOT PULL ON POWER CORDS!
2. Gently lift the gel tray from the unit. Al-
ways wear gloves, eye protection and
protective clothing if buffer and /or gel
contain Ethidium Bromide. Ethidium Bro-
mide is a powerful mutatgen; gloves, eye
protection and protective clothing should
always be worn when handling the gel or
buffer solutions. View separated fragments
under UV light, using proper protection for
eyes and skin (see manufacturer’s instruc-
tions). Alternatively, Safe Stains may be
used and viewed using blue light or UV
excitation.