C.B.S. Scientific GSL-1001 - Manual (Page 3)

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PREPARATION AND POURING OF SEQUENCING GELS USING THE GEL SLIDER™
Advance preparation:
Prepare solutions, level Gel Slider, pre-coat notched glass plate, and make sure you have completed step number C-1
(page 1 of these instructions), so that side adjustment panels are correct according to the thickness of your back glass
plate (either 1/8” thick or 3/16” thick).
Step 1. Adjust width of Gel Slider by turn-
ing over and placing glass plate into
recessed area in bottom of side panels
as shown in photo 1. Adjust width
by loosening black thumb screws and
allowing side panels to increase or
decrease width of unit until plate fits
snuggly.
Step 2. Place back glass plate onto Gel Slider
up against back plate stops. Glass plate
stops are factory installed at the 42cm
position. The stops can be moved
to 22cm and 32cm position if cast-
ing shorter gels. Position spacers and
clamp to back glass plate as shown.
Place notched plate (coated side down)
at bottom of back plate, as shown in
photo2.
Step 3. Add catalyst to acrylamide solution
and fill syringe. Start casting by filling
overlapping area between plates and
beginning to slide top plate. Elapsed
time: 2:03 minutes.
notched plate
Spacers
back plate stops