C.B.S. Scientific EBU-6000 - Manual (Page 5)

www.cbsscientific.com 5 Semi-Dry Blotter Manual 12/12
SECTION 1
General Information
1.1 Introduction
The C.B.S. Scientific Semi-Dry Blotting Systems will transfer Western, Northern, or Southern
blots reliably and quickly. These blotting systems have transfer capabilities for gel dimensions up
to 12cm x 34cm (Cat. # EBU-3000), 20cm x 20cm (Cat. # EBU-4000) or 35cm x 45cm (Cat. #
EBU-6000). The stainless steel cathode and platinum-coated titanium anode are durable and
corrosion-resistant. These electrodes create a uniform electric field producing excellent transfers
onto nitrocellulose, supported nitrocellulose, nylon, charged nylon or activated paper substrates.
The upper electrode housing is separate from the base, allowing it to be secured at the desired
height with side hand screws. This unique design permits several gels to be transferred
simultaneously.
In the Semi-Dry system, blotting is accomplished by layering transfer membrane and gel between
buffer saturated blotting paper, minimizing the amount of buffer required. To prevent drawing
current away from the gel during a transfer, the uncovered portion of the cathode is shielded with
a Mylar® mask. This shield insures that all applied current passes directly through the gel. Use
of the mask will increase transfer efficiency and reduce transfer times up to 50%.
1.2 Specifications
Constructions:
Buffer chamber, safety cover Acrylic
Electrode panels Stainless steel, platinum-coated titanium
Electrode support panel Styrene
Power cords FR Urethane rated 7500VDC @ 200mA,
65°C
Mylar masks Mylar
EBU-3000 EBU-4000 EBU-6000
Shipping
weight
7lbs 7lbs 15lbs
Overall Size
(l)x(w)x(h) cm
15 ¾ x 7 ¾ x 2 ¾ 9 ¾ x 9 ¾ x 2 ¾ 19 ¾ x 17 x 2 ½
Maximum Gel Size - cm 12cm x 34cm 20cm x 20cm 35cm x 45cm
Distance between
electrodes -cm
1cm 1cm 1cm
Recommended Power
Supply EPS-600 EPS-600 EPS-600
1.3 Safety
Power to the Semi-Dry Blotter is to be supplied by an external DC voltage power supply that must
be ground isolated so that the DC voltage output floats with respect to ground. For any power
supply used, the maximum specified operating parameters for the units are:
Maximum Limits
EBU-3000 EBU-4000 EBU-6000
50 VDC 50 VDC 50 VDC
500mA current 500mA current 1200mA current
55°C ambient temperature 55°C ambient temperature 55°C ambient temperature
Current to the unit, provided from the external power supply, must enter the unit through the lid
assembly, providing a safety interlock to the user. Current to the unit is broken when the lid is
removed. Do not attempt to use the unit without the safety lid, and always turn the power
supply off before removing the lid, or when working with the unit in any way. Follow
safety precautions specified by the power supply manufacturer.