C.B.S. Scientific EBU-402 - Manual (Page 5)

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Double & Triple-Wide Mini-Blotters Instruction Manual Oct 2012
SECTION 1
General Information
1.1 Introduction
Electroblotting is a fast, easy, and efficient technique for transferring proteins from PAGE gels
nitrocellulose and nucleic acids from agarose/PAGE gels onto nylon transfer membranes. The
Wide Mini-Blotting Systems can effectively complete transfer in less than 2 hours compared to 6
to 24 for the more traditional “capillary” transfer. The C.B.S. Scientific Electrophoretic Blotting
Systems come equipped with two electrode panels, one color-coded gel cassette assembly,
holding tank with cooling chamber and exit ports and safety cover with attached power leads.
The EBU-302 was designed primarily in conjunction with the Triple Wide Mini-Vertical Gel System
(MGV-102/202-33), although the gel cassette will accommodate gels smaller than the set
dimensions of 9cm (H) x 32.8cm (W) and can transfer up to two gels at one time. The EBU-402
was designed for use with the MGV-202-20 Double-Wide Mini-Vertical System, and can transfer
two double wide gels with 20 x 10cm dimensions, or four standard mini gels.
1.2 Specifications
Constructions:
Buffer chamber, safety cover Acrylic
Electrode panels Acrylic and platinum wire .012" diameter
Power cords FR Urethane rated 7500VDC @ 200mA,
65°C
Gel cassette Perforated fiberglass panels red and
black, two Scotch-Brite® pads, one
sponge pad
Safety Certification EN61010-1-1993 (IEC1010-1)
EBU-402 EBU-302 EBU-362
Shipping
Weight
7lbs 9lbs 11lbs
Overall Size
(l)x(w)x(h) cm
24 x 9.5 x 16 38 x 10 x 16 39 x 9 x 22
Maximum Gel Size – cm
(w) x (h)
20 x 9 32.8 x 9 32 x 13
Distance between
electrodes –cm
5.3cm 5.5cm 5.5cm
Recommended buffer
volume
1 cassette
2 cassettes
1.5L
1.4L 2L
1.9L
3L
2.8L
1.3 Safety
Power to the Electro-Blotter is to be supplied by an external DC voltage power supply that must be
ground isolated so that the DC voltage output floats with respect to ground. For any power supply used,
the maximum specified operating parameters for the units are:
Maximum Limits
100 VDC
150 watts power
1500mA current
50°C ambient temperature
Current to the unit, provided from the external power supply, must enter the unit through the lid
assembly, providing a safety interlock to the user. Current to the unit is broken when the lid is
removed. Do not attempt to use the unit without the safety lid, and always turn the power
supply off before removing the lid, or when working with the unit in any way. Follow
safety precautions specified by the power supply manufacturer.