C.B.S. Scientific EBU-402 - Manual (Page 11)

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Double & Triple-Wide Mini-Blotters Instruction Manual Oct 2012
SECTION 5
Maintenance of Equipment
5.1 Care and Handling
The plastic components of the Electrophoretic Blotting units are fabricated from acrylic and
polycarbonate. Electrodes and connectors are made from pure platinum, stainless steel, and
chrome plated brass. As with any laboratory instrument, adequate care ensures consistent and
reliable performance.
After each use, rinse buffer chamber, gel tray and combs with de-ionized water. Wipe dry with a soft
cloth or paper towel, or allow to air dry. Whenever necessary, all components may be washed
gently with water and a non-abrasive detergent, and rinsed and dried as above. Never use abrasive
cleaners, glass cleaning sprays or scouring pads to clean the components, as these will damage the
unit and components.
Additional precautions:
• Do not autoclave or dry-heat sterilize the apparatus or components.
• Do not expose the apparatus or components to phenol, acetone, benzene,
halogenated hydrocarbon solvents or alcohols.
• Avoid prolonged exposure of the apparatus or components to UV light.
• For Northern blotting, DO NOT treat with diethylpyrocarbonate (DEPC)-treated
water for extended periods at 37°C.
• Electrostatic charge and heat affix the conductive platinum surface covering the
titanium. It can be scratched off or damaged by using sharp objects or scouring
pads. This will disturb the continuity of the electric field and have a negative
effect on transfers.
• The stainless electrode can be permanently damaged by corrosion if the polarity
between anode and cathode is reversed. CAUTION: Be certain of power
connections before initiating transfer.
5.2 Maintenance
The following inspection and maintenance procedures will help maintain the safety and
reliable performance of the Blotting systems. Replacement parts can be ordered by
calling 858-755-4959 or by contacting your local distributor.
• Banana plugs and power cords should be inspected regularly. If the banana
plugs become loose or do not feel friction tight replace the plugs or power cords.
• Should power cord assemblies (connectors, wire or shrouds) show any signs of
wear or damage (e.g. cracks, nicks, abrasions, or melted insulation), replace
them immediately.
• The platinum wire is secured to the banana jack by compression between a
stainless washer and the jack nut. The nut/washer interface should be tight and
free of corrosion.