C.B.S. Scientific EBX-700 - Manual (Page 9)

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5. Assemble blotting stack as shown below. With cassette wide open assemble components
on black side in the following order: buffer saturated sponge pad, gel equilibrated in transfer
buffer, buffer saturated transfer membrane, then buffer saturated blotting paper. Smooth with
gloved fi nger or roll with glass rod to be sure no bubbles exist between the gel and the transfer
membrane.
6. Insert blotting cassettes into the lower reservoir. Red side of cassette should face
toward red electrode panel.
7. Place 2 freezer blocks into the space provided in the lower reservoir. Depending on transfer
conditions buffer temperature rise can be limited by exchanging freezer blocks half way
through run.
red side
blotting paper
gel
foam pad
black side
membrane