4 operation, Operation, Ept.i.p.s.® — instructions for use – Eppendorf epT.I.P.S. Version A User Manual
Page 5
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epT.I.P.S.® — Instructions for use
5
4
Operation
4 Operation
You will find recommendations for handling in the operating manual for the instrument you are
using.
The dispensing techniques used most frequently are forward pipetting, reverse pipetting,
dispensing, sequential dispensing and diluting. Mechanical pipettes are only suitable for forward
and reverse pipetting.
Irrespective of the dispensing technique use, the following items should be taken into
consideration during pipetting:
•
When aspirating the liquid, the tip should only be immersed a few millimeters into the
medium.
•
The filled tip should be moved up against the wall of the vessel to avoid residues of liquid on
the outside of the tip.
•
Pre-wetting the tip up to three times will improve the accuracy and precision of the results.
•
Liquid should be aspirated slowly and evenly.
•
A waiting period of 3 seconds should be allowed for the liquid to rise in the tip.
•
The pipette should be held vertically during aspiration.
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