Eppendorf Maxipettor 4720 - Manual (Page 11)

Operation
Varipette ® 4720
English (EN)
11
4.2 Setting the volume
4.3 Pre-wetting
Precise pipetting requires the preliminary wetting of the Varitip P and the Maxitip.
 Aspirate and dispense liquid three times.
The tip is pre-wetted.
After pre-wetting, the amount of dispensed liquid corresponds exactly to the previously
aspirated volume that was set.
4.4 Pipetting with the Varitip P
4.4.1 Aspirating liquid
1. Immerse the tip of the Varitip P in the liquid.
2. Slowly press down the pipetting lever up to the lower stop.
3. Wait for 2 seconds before letting the pipetting lever slide upward slowly.
4.4.2 Liquid dispensing
You can dispense the liquid from the Varitip P freely or against the inner wall of the vessel.
1. Slowly press down the pipetting lever until it reaches the lower stop.
2. Remove any residual droplets left behind on the tip by wiping the tip on the vessel wall.
3. Let the pipetting lever slide back slowly to the upper stop.
Abb. 5:Setting the volume
Fig. 5: Setting the volume
 Turn the selection dial until the required volume is
displayed. The individual digits of the volume
display must entirely show in the viewing window.
The volume is displayed in mL in 4 digits with two
decimal spaces.
A small air bubble is left behind in the cylinder of the Varitip P after filling. This has
no effect on the accuracy and precision of dispensing.
Liquids remaining in the Varitip P after pre-wetting and pipetting are not part of the
dispensing volume.