Eppendorf Microinjection of cDNA into Drosophila embryos - Manual (Page 4)

Userguide No
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Ordering information
Product Description Order no.
International
Order no.
North America
FemtoJet® Programmable microinjector with integrated pressure supply 5247 000.013 920010504
Femtotip II™
20 sterile glass capillaries for microinjection into adherent and
suspension cells
5242 957.000 930000043
TransferMan® NK 2 Micromanipulator for suspension cells 5188 000.012 920000011
InjectMan® NI 2 Dynamic micromanipulator for microinjection 5181 000.017 920000029
Microloader™ Capillaries for filling Femtotips, 2 racks of 96 5242 956.003 930001007
Microcentrifuge
Eppendorf 5424
with rotary knobs, includes aerosol-tight 24 x 1.5/2.0 ml rotor
and lid
5424 000.410 022620401
[1] Bischof J, Maeda RK, Hediger M, Karch F, Basler K (2007) An optimized transgenesis system for Drosophila using
germ-line-specific phiC31 integrases. Proc Natl Acad Sci U S A 104(9):3312-7.
[2] Thorpe HM, Smith MC (1998) In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the
resolvase/invertase family. Proc Natl Acad Sci U S A 12;95(10):5505-10.
[3] Groth AC, Fish M, Nusse R, Calos MP (2004) Construction of transgenic Drosophila by using the site-specific integrase
from phage phiC31. Genetics 166(4):1775-82.
[4] Thorpe HM, Wilson SE, Smith MC (2000) Control of directionality in the site-specific recombination system of the
Streptomyces phage phiC31. Mol Microbiol 38(2):232-41.
[5] Spradling AC, Rubin GM (1982) Transposition of cloned P elements into Drosophila germ line chromosomes.
Science 218(4570):341-7.
References