3 operation 3, Operation – Eppendorf 5702 RH Centrifuge User Manual
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The fixed-angle rotor F-35-30-17 can be loaded with 15 ml Falcons
®
and 15 ml round bottom centrifuge tubes. It is
available in the 10 and 30 position versions. Please use only swing-bucket rotor A-4-38 with round buckets and the
appropriate adapters for centrifuging blood sampling systems.
Before centrifuging the Falcons
®
, place their plastic adapters in the tube holders. The adapters prevent the Falcons
®
from
being destroyed. For the centrifugation of round bottom tubes made of Duran
®
glass, round rubber pads are required to
prevent the tubes from cracking. Make sure a rubber pad is placed under each glass tube before centrifuging.
In order to avoid breakage of the tubes when using centrifuge tubes of glass, polystyrene or similar materials, please
observe the limiting data with reference to endurance specified by the manufacturer.
The maximum load capacity for Falcons
®
is 20 x 15 ml; for 15 ml round bottom centrifuge tubes 30 x 15 ml. The rotor can
be loaded with a combination of 15 ml Falcons
®
and 15 ml round bottom centrifuge tubes. The maximum capacity is then
20 x 15 ml Falcons
®
and 10 x 15 ml round bottom centrifuge tubes.
Fig.3: Loading of rotor F-35-30-17 with 15 ml Falcons
®
and 15 ml round bottom centrifuge tubes
The specified max. weight of 56 g imprinted on the rotor is the gross weight rating including the tube holder,
adapter, tubes and contents. Always weigh the tube holder and the load together.
A maximum of 24 1.5/2.0 ml test tubes can be centrifuged in rotor F-45-24-11. If the appropriate adapters are used, it is
also possible to load it with 0.2 ml PCR tubes, 0.4 ml test tubes, 0.5 ml test tubes and 0.6 ml Microtainers
®
.
Fixed-angle rotor F-45-18-17-Cryo can be equipped with cryotubes (max. diameter 17 mm or 13 mm with adapters) and
sealable centrifuge tubes (max. diameter 16.5 mm or 12.2 mm with adapters). In each of these cases, max. tube length is
50 mm.
Maximum load (adapter, tube and contents) per bore is 3.75 g on rotor F-45-24-11 and 8.7 g on rotor F-45-18-17-Cryo.
When loading the rotors, please make sure that the tubes are inserted in the bores of the rotor opposite one another in
pairs.
To ensure that the rotor is loaded symmetrically, the tubes opposite one another need to contain approximately the same
filling quantity.
Fixed-angle rotors are operated without a rotor lid.
3.4 Fixed-angle rotor
15 ml Falcon
®
15 ml
round bottom
centrifuge tube
Operation
3 Operation
3