Next Advance DX - Manual (Page 7)

O
PERATOR
’
S
M
ANUAL FOR THE
F
REEDOM
R
OCKER
™ DX
7
Dispensing Antibodies
To dispense small quantities, e.g. 10
mL of antibodies or other precious
reagents, fill up the 50 mL tube as
the source container, loosen the
cap, and set the fill volume to 30 mL
and set the clearing rinse on. The
clearing rinse is preset to empty the
tubing of the last reagent/buffer.
Setting the dispense to 30 mL
makes sure that enough air is
pumped through the tubing after the
50 mL of antibody, so that the
antibody is pumped through the
tubing and into the trays.
For example, to dispense 10 mL to
each of the trays, fill the source
container with 50 mL and set the fill
volume to 30 ml. The clearing rinse
will use up approximately 4 mL (not
recoverable). The rest of the
antibody solution will then be
dispensed into the trays. Allow a
little extra since some trays might
receive 1 mL more than others. Run or Save Program
Once you have set the parameters
for all the steps you want in your
program you can save the program
or just run it.
To run the program without saving
it, just push START. To save this
program, push the left arrow key
until you are at the top level, then
push the up or down arrow keys
until you are at Save Program.
Then rotate the knob to select the
desired program number. You can
then begin the program by pushing
START.
While the program is running,
parameters can be changed by
pressing PAUSE, editing the
program parameters, and then
pressing START/PAUSE again.
These changes will affect future
operations, but not the operation in
progress. However, you can change
the current rocking speed by
rotating the jog wheel.
Table 1. Sample Program - overnight Western antibody staining and washing
7
DRAIN Description
FROM VOLUME TIME SPEED TO
(1 to 9) (yes or no)
(white, blue,
y'low)
(0 to 600 ml)
(hr:min, from
:00 to 90:00)
(30 to 100)
(red, black, or
green)
1 100 2:00 30 red blocker in trays
2 3 yes white 100 :20 80 red 3X 20 min wash
3 1 yes blue 30 7:00 30 black
10 mL Ab (use 50 mL tube,
clearing rinse) and recover
4 3 no white 100 :05 100 red 3X 5 min wash
5 2 yes white 200 :30 80 red 2x 30 min wash
6 1 no yellow 50 1:30 30 green
50 mL secondary Ab,
recover
7 3 yes white 100 :30 80 red 3X 30 min wash
8 not used
9 not used
ROCK
No. of Steps (up to 9)
STEP REPS
CLEARING
RINSE
FILL