Next Advance DX - Manual (Page 6)

O
PERATOR
’
S
M
ANUAL FOR THE
F
REEDOM
R
OCKER
™ DX
6
Rocking commences with a count-
up timer. If the cursor were
blinking next to the countdown
timer value, then Rocking would
commence with a count-down timer.
The Rocking speed may be adjusted
“on-the-fly”, using the jog wheel.
The minimum speed is 30% and the
maximum is 100%.
Automatic Mode, Step 1
In Automatic Mode, the Freedom
Rocker™ follows a programmed set
of steps. Each step is similar to a
step in a protocol. Select Edit Auto
Steps from the top menu.
Press the right arrow and use the
jog wheel to select the number of
steps in your protocol. (This value
can be revised later.) Then push
the down arrow once to go to the
first step (or push it repeatedly to
cycle through the steps). Then as in
manual mode, push the right arrow
to proceed to select the values of
the salient parameters.
The first step in any Automatic
mode program only specifies four
parameters: Rocking speed,
Rocking duration, Drain volume
and Drain container. The right
arrow will allow you to scroll
through these parameters. Use the
jog wheel to change the settings.
The 4 parameters are now set for
step 1. Push the left arrow to go up
a level. You will see the step
number displayed.
Auto Mode, Steps 2 and Up
You can move to the next step
number by pressing the down key.
Subsequent steps specify more
parameters: the parameters in step
1 plus the number of repetitions,
whether a clearing rinse is desired,
the Fill volume, and Fill source.
Next cycle through the parameters
with the right arrow. Step 2 and the
rest of the steps can have a clearing
rinse and multiple repetitions.
Clearing Rinse
The clearing rinse precedes each fill
operation for optimal washing or to
clear the tube set before a different
reagent is used. The default
condition is with the clearing rinse
present. For example, before
dispensing a reagent (up to 50 mL
per tray), the clearing rinse is set to
clear the tube set of any buffer
remaining from the prior step. For
more than a 50 mL
dispense/aspirate step, the clearing
rinse will dispense enough buffer to
rinse the bottom of the trays and
greatly reduce the amount of
residual reagent from the previous
step. In most all cases it is
beneficial to incorporate the clearing
rinse.
Multiple Repetitions
The multiple repetitions feature
enables you to do a series of similar
rinses. For example, to do a series
of 3 rinses, set the number of
repetitions to 3.