Sper Scientific 300116 Fixed Volume Pipettor - Manual (Page 3)

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III. MEASUREMENT PROCEDURES
1. Secure a pipette tip to the TIP of the unit.
2. Holding the unit vertically, depress the PLUNGER to the first stop. Next
fully immerse the TIP 2 to 4 mm into the solution.
3. Slowly release the PLUNGER until it returns to the original position.
4. Remove the unit from the solution after 1 to 2 seconds.
5. To discharge the solution from the pipette tip, place the TIP against the
inside wall of the receptacle. Depress the PLUNGER to the first stop,
then depress fully to completely blow out the solution from the TIP.
6. Next move the TIP up and along the inner wall of the receptacle several
times.
7. You may now remove the unit and release the PLUNGER.
8. Depress the TIP EJECTOR to remove the pipette tip.
9. Clean the unit using an autoclave or ultrasonic cleaner as required by
your application. The unit may be autoclaved for a maximum of 8 times,
after which, the unit should be discarded. Calibration remains stable
after occasional autoclaving. Annual recalibration by a professional
service center is recommended to ensure accuracy.
Note: Inserting objects into the top of the tip assembly can damage the
pipette seal.
Product
Number
Range ( µl ) Accuracy (%) Repeatability (%)
300107 5 µl ± 4.00 < 2.80
300108 10 µl ± 2.5 < 1.00
300109 20 µl ± 2.0 < 1.00
300110 25 µl ± 2.0 < 1.00
300111 50 µl ± 2.0 < 1.00
300112 100 µl ± 1.2 < 0.50
300113 200 µl ± 1.0 < 0.40
Specifications
300114 500 µl ± 1.0 < 0.40
300115 1000 µl ± 1.0 < 0.30
300116 5000 µl ± 0.8 < 0.20