Sper Scientific 300104 Adjustable Pipettor - Manual (Page 3)

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III. MEASUREMENT PROCEDURES
1. Gently pull upwards on the ADJUSTABLE VOLUME WHEEL and turn
it in either direction until the desired setting appears in the DIGITAL
DISPLAY (see chart below).
DIGITAL DISPLAY Settings:
300100 300101 300102 300103
1 0 1 0
2 7 2 7
5 5 5 5
1.25µl 7.25µl 12.5µl 75µl
300104 300105 300106
1 0 0
2 7 2
5 5 5
125µl 0.75ML 2.5ML
2. To lock in your desired volume, push the ADJUSTABLE VOLUME
WHEEL back down into the locked position. The small grooves in the
bottom of the WHEEL align with the handle.
3. Secure a pipette tip to the TIP of the unit.
4. Holding the unit vertically, depress the PLUNGER to the first stop. Next
fully immerse the TIP 2 to 4 mm into the solution.
5. Slowly release the PLUNGER until it returns to the original position.
6. Remove the unit from the solution after 1 to 2 seconds.
7. To discharge the solution from the pipette tip, place the TIP against the
inside wall of the receptacle. Depress the PLUNGER to the first stop,
then depress fully to completely blow out the solution from the TIP.
8. Next move the TIP up and along the inner wall of the receptacle several
times.
9. You may now remove the unit and release the PLUNGER.
10. Depress the TIP EJECTOR to remove the pipette tip.
11. Clean the unit using an autoclave or ultrasonic cleaner as required by
your application. The unit may be autoclaved for a maximum of 8 times,
after which, the unit should be discarded. Calibration remains stable
after occasional autoclaving. Annual recalibration by a professional
service center is recommended to ensure accuracy.
Notes:
•
Highly concentrated solutions may require increased or decreased
DIGITAL DISPLAY settings. Make adjustments as needed.
• Inserting objects into the top of the tip assembly can damage the pipette
seal.