Carolina C-FERN Express Extended Culture - Manual

C-FERN Express ™
Extended Culture Instructions
C-FERN Express ™ is a new strain of C-FERN ® that has been developed to
enhance student investigations through to the fertile sporophyte stage of
the life cycle. Derived from two Japanese varieties of Ceratopteris
thalictroides, C-FERN Express exhibits very rapid gametophyte and
sporophyte development. It can be substituted for the wild-type strain
(RNWT1) in student investigations of the fern life cycle. Culture
requirements for gametophytes are essentially the same as with the
standard C-FERN strains. Slight developmental differences in
gametophytes are noted below. In addition, the following guidelines for
sporophyte culture will allow studies of the entire life cycle, from spore to
spore, within 60 days.
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10-day gametophyte culture.