Uv transilluminator function – Hoefer UV20 User Manual
Page 9

UV transilluminator
function
The Hoefer
®
MacroVue Dual-intensity UV
transilluminator provides a uniform and intense
source of 302 nm ultraviolet light to back-
illuminate transparent fluorescent materials.
Nucleic acids stained with fluorescent dyes
such as ethidium bromide and acridine orange
can be detected under UV illumination. This
visualization method is especially suitable for
double-stranded nucleic acids, but is less sensitive
for visualizing single-stranded nucleic acids.
The
MacroVue UV-20
filter surface is 20 ×
20 cm. The light intensity level can be set to
either high or low. The transilluminator can be
converted from 302 nm (medium wavelength) to
254 nm (short wavelength) by replacing the UV
lamps as described on page 6.
•
p1
Fig 1. MacroVue UV-20
Transilluminator main
components.
Dual intensity switch
(high and low)
Illumination
area (filter)
Mains power
switch
Hinged UV-blocking
safety cover