Physitemp Instruments BFS 60MP - Manual (Page 4)

4
Before the surface of the stage freezes apply an ample amount of water/OCT/
sucrose solution to form a base onto which you can freeze your specimen. Once the
specimen has become attached freezing will be facilitated by building a dam of
liquid around the sides of the specimen to reduce heat losses from the specimen to
the surroundings. When the specimen is thoroughly frozen move the microtome
sledge slowly back and forth under the knife to ensure that none of the wiring or
water tubes are interfering with the operation of the microtome.
Quick Thaw feature
In order to quickly remove a frozen specimen from the stage, depress the white
spring loaded on the front panel to the ‘THAW’ position and hold it there until the
surface of the stage melts. Release the switch and quickly remove the specimen
before the surface refreezes. Do not depress the switch longer than is necessary to
remove the specimen as this may cause the stage surface to overheat.
Safety Features
A protection device built into the cooling stage disconnects power to the stage in
the event that the water supply is not turned on or becomes disrupted or when the
plate surface temperature exceeds 60 degrees Centigrade. In the event that either of
these conditions should occur a warning lamp labeled ‘Fault Condition’ will be
illuminated on the front panel. When the condition is rectified the lamp will
extinguish.
In the event of a short circuit in any of the four independent power supplies in the
controller shut off the AC power for 10 seconds and then reconnect. If the problem
persists please contact our engineering department at 1-800-452-8510 between the
hours of 9am-5pm EST.