ANKOM HCl Series - Manual (Page 13)

Operator’s Manual
Rev L 11/12/14 pg. 13
Hydrolysis Procedure
Total Fat Calculation
The hydrolysis procedure is done prior to fat extraction. When both hydrolysis and fat extraction are done, the Total
Fat contained within a food or feed sample can be calculated using the following formula:
% Total Fat = 100 x (W
2
– (W
3
+ (C
1
– C
2
)))
W
1
Where: W
1
= Original weight of sample
W
2
= Weight of dried sample, filter bag, and Diatomaceous Earth (DE) after hydrolysis
W
3
= Weight of dry extracted sample, filter bag, and DE
C
1
= Blank filter bag dry weight after hydrolysis
C
2
= Blank filter bag weight after extraction
Hydrolysis Support Items
The following support items are needed to perform the hydrolysis procedure:
Item Recommended Product
Electronic Balance with four-place readout ANKOM #TB Balance Hardware
ANKOM #TBS Balance Software
Filter Bags ANKOM #XT4
Bag Holder (used for adding sample to an empty filter bag) ANKOM #101.2
Diatomaceous Earth (DE) ANKOM #XTC
Heat Sealer for sealing the filter bags ANKOM #1915 (120V), #1920 (220V)
Solvent Resistant Marker ANKOM #F08
Multi-bag Holder (used to hold bags in the Hydrolysis Chamber) ANKOM #H33
Desiccant Pouch for drying ANKOM #X45
Oven for drying ANKOM #RD (120V), #RDI (220V)
Sample
Spoon