Bio-Rad Aurum Serum Protein Mini Kit - Manual (Page 8)

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11. Centrifuge the column for 20 sec at 10,000 x g in a microcentrifuge,
collecting the eluate in the “unbound” 2.0 ml collection tube.
12. Remove the column and tube together from the centrifuge and wash the
resin with 200 µl of the binding buffer. Replace tube and column in
centrifuge.
13. Centrifuge column for 20 sec at 10,000 x g in a microcentrifuge, collecting
the eluate in the same “unbound” tube, which contains the albumin- and
IgG-depleted serum sample.
14. The treated sample is ready for IEF analysis. For serum, this procedure
typically yields protein concentrations around 1.5–2.0 mg/ml as determined
by the modified Lowry method (Bio-Rad DC protein assay, catalog #500-
0112).
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