Bio-Rad ReadyPrep Protein Extraction Kit (Signal) User Manual
Page 5
Parton and Simons 1995, Anderson et al. 1992). Due to
the lipid composition of these membrane subdomains,
these proteins become resistant to solubilization at 4°C
with many nonionic detergents such as Triton X-100 and
can be selectively recovered from the detergent-insoluble
membrane fraction. Based upon the solubility properties of
these signaling proteins, the ReadyPrep protein extraction
kit (signal) utilizes a proprietary mixture of nonionic
detergents to extract and remove hydrophilic and soluble
proteins, leaving behind the signal proteins in a detergent-
insoluble fraction. After the fractionation procedure has
been completed, the signal proteins are solubilized in the
protein solubilization buffer (PSB) supplied with the kit. The
solubilized proteins can then be immediately used in 2-D
gel electrophoretic applications.
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