Bio-Rad ReadyPrep™ Protein Extraction Kit (Membrane I) - Manual (Page 11)

4.2 Processing Hydrophilic and Hydrophobic
Membrane Protein Fractions for IEF/2-D Analysis
10. Determine the protein concentration of the hydrophilic
and hydrophobic membrane protein fractions. The RC
DC Protein Assay is recommended. This assay allows
accurate protein quantitation in the presence of
detergents, reducing agents, and other substances
that typically interfere with other protein assays.
11. Process an appropriate amount of the hydrophobic
membrane protein fraction, and if desired, hydrophilic
protein fraction through the ReadyPrep 2-D cleanup
kit. To determine an appropriate amount, refer to the
guidelines in Section 5.1. Follow the ReadyPrep 2-D
cleanup kit protocol provided. At the end of the 2-D
cleanup protocol, resuspend the protein pellet in
complete PSB (See Section 5.2 for preparation
instructions) and then perform IEF/2-D analysis.
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