Bio-Rad ReadyPrep™ 2-D Cleanup Kit - Manual (Page 18)

5.2.2.2. Protein Solubilization Buffer (PSB).
Protein solubilization buffer (PSB) and PSB diluent are
provided with ReadyPrep protein extraction kit
(membrane I) and ReadyPrep protein extraction kit
(cytoplasmic/nuclear) to solubilize the protein pellet after
using the ReadyPrep 2-D cleanup kit. PSB is a proprietary,
strongly chaotropic 2-D rehydration/sample buffer that will
solubililze both hydrophilic as well as hydrophobic proteins.
To make 2 ml of complete 2-D rehydration/sample buffer,
add 1.1 ml of PSB Diluent to each 1 g of PSB powder.
Note: Before weighing out the PSB powder, shake the bottle
vigorously for 10-15 sec to break up any clumps and to ensure
a uniform blend of the different components.
Mix the solution until the powder is completely dissolved
(the tube can be warmed to speed dissolution of the solids,
but do not allow the temperature to exceed 30°C). Add
DTT, Bio-Lyte ampholyte and Bromophenol Blue as
directed in Section 5.2.2.1 to complete the preparation of
the Buffer.
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