Bio-Rad ProteoMiner Protein Enrichment Kits - Manual (Page 3)

Section 1
Introduction
The ProteoMiner™ technology is a novel sample preparation tool used for the
compression of the dynamic range of the protein concentration in complex
biological samples. High-abundance proteins present in complex biological
samples like sera or plasma, make the detection of medium- and low-
abundance proteins extremely challenging. This technology provides a method
of overcoming this challenge, allowing for the exploration of the entire
proteome.
This is accomplished through the use of a large, highly diverse bead-based
library of combinatorial peptide ligands. When complex biological samples are
applied to the beads, the high-abundance proteins saturate their high affinity
ligands and excess protein is washed away. In contrast, the medium- and low-
abundance proteins are concentrated on their specific affinity ligands. This
reduces the dynamic range of protein concentrations while maintaining
representatives of all proteins within the original sample.
Section 2
Kit Specifications
ProteoMiner™ Small-Capacity Kit (Catalog #163-3006)
Provides reagents for processing 10 samples. Compatible with 2-D gel
electrophoresis and other downstream protein separation analysis methods.
• Spin Columns. 10 columns each containing 500 µl bead slurry (4% beads,
20% v/v aqueous EtOH), 20 µl settled bead volume
• Wash Buffer. 50 ml PBS buffer (150 mM NaCl, 10 mM NaH
2
PO
4
, pH 7.4)
• Elution Reagent. 2 vials, lyophilized urea CHAPS (8 M urea, 2% CHAPS)
• Rehydration Reagent. 5 ml, 5% acetic acid
• Collection Tubes. 20 capless, 2 ml centrifuge tubes; 10 capped, 2 ml
centrifuge tubes
• Instruction Manual
• Quick Start Guide
ProteoMiner Large-Capacity Kit (Catalog #163-3007)
Provides reagents for processing 10 samples. Compatible with 2-D gel
electrophoresis and other downstream protein separation analysis methods.
• Spin Columns. 10 columns each containing 500 µl bead slurry (20%
beads, 20% v/v aqueous EtOH), 100 µl settled bead volume
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