Labnet Biopette A Autoclavable Multichannel Pipettes - Manual (Page 6)

M U L T I C H A N N E L
6
to check whether the following requirements
have been fulfilled during error determination:
• the ambient temperature, and the temperature
of the pipette, tips and water was identical
• the density of the liquid used is close to that
of distilled water
• a balance with appropriate sensitivity has been
used
• mg/µl conversion factor has been taken into
account
• the requirements given in sections 3 and 4
have been fulfilled
If the above conditions are satisfied and the accu-
racy error for selected volume given in section 1
exceeds the permissible value, the pipette
recalibration procedure should be carried out.
The recalibration can be performed within one
full turn of the key to the right or to the left only.
Recalibration conditions:
• Ambient temperature and the temperature of
the pipette, tips and liquid should be within
the range 20-25°C and stabilized during weigh-
ing within ±0.5°C
• Measurements should be conducted using
distilled water
• Balance sensitivity should be suitable for the
volume to be controlled
Recalibration procedure:
• Set the dose volume depending on the pipette
volume according to the following table:
5
4 - RECOMMENDATIONS
To achieve maximum safety, precision and re-
liability, the following principles should be ob-
served:
• do not draw liquids without tips fitted on the
pipette cones,
• do not lay down the pipette with tips filled,
• do not draw volumes of liquid exceeding the
pipette’s range,
• check if the tips are fitted properly,
• during operation, the pipette must be held ver-
tically, tips should be immersed in liquid
to the depth of 2 to 4 milimeters and the pipet-
ting button should be depressed and released
slowly and evenly,
• new tips must be pre-rinsed prior to pipetting,
by drawing and expelling the liquid to be me-
asured. This is especially important when wor-
king with liquids of viscosities and densities
different from that of water and in temperatu-
res different from ambient temperature,
• when pipeting liquids which tend to wet walls
of tips, such as serums, proteins or organic
solvents, measuring must be performed much
slower than with other liquids,
• tips must be replaced with new ones, when
changing from one liquid to another, or if drops
of liquid remain inside the tips,
• after work, the pipette should be stored verti-
cally in a holder with tips removed.
5 - RECALIBRATION
BioPette™ pipettes are calibrated by gravimetric
method, using Labnet tips and distilled water, at
the temperature 20±1°C, according to EN ISO
8655 standard.
If during pipette operation you find that the
accuracy error (the difference between the real
aspirated volume and the preset volume)
exceeds the permissible value given in the table
in section 1, the pipette recalibration procedure
should be carried out.
Before starting the recalibration it is necessary
Volume checked [µl]
0.1 - 10
10 - 100
> 100
Balance sensitivity [mg]
≤ 0.001
≤ 0.01
≤ 0.1
1 - 10
5 - 50
20 - 200
50 - 300
1
5
20
50
0.92 - 1.08
4.8 - 5.2
19.6 - 20.4
49.2 - 50.8
0.33
1.67
6.30
10.00
Range
of the pipette
volumes [µl]
Preset
volume
[µl]
Permissible
volumes
[µl]
Volume change ∆V for
full turn of the calibra-
tion key [µl]
(24 increments)
BPM/08/2004 v06 31/07/2004 01:06 PM Page 10