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Lubrication, Decontamination/disinfection – Eppendorf White Paper 14 User Manual

Page 5

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WHITE PAPER I No. 14 I Page 5

Lubrication

After cleaning your equipment take a small amount of
centrifuge lubricant (e.g. glycerol, talcum or peg fat from
Eppendorf, order number 5810 350.050) onto your finger.
Lubricate bucket grooves, pivots of swing-bucket rotors, and
rubber seals (Fig. 5). Check if seals of aerosol tight lids/caps

Decontamination/Disinfection

Even when working accurately, a contamination of the rotor
by biological (for example blood) or radioactive material
may happen. Please consult your laboratory safety officer
first about suitable methods of cleaning and disinfecting any
hazardous spill within the centrifuge/rotor. Successful
disinfection can only be granted by the suppliers of the
chemicals. Before using any cleaning or disinfection method

need to be replaced. Aerosol-tightness is limited to
undamaged seals. Lubricate the threads of the fixed angle
rotors after cleaning and autoclaving. Light waxing of the
rotor surface may also extend the life-time of the anodized
surface. Use a soft cloth for waxing procedure.

Figure 5: Lubrication of bucket grooves and pivots of swing-bucket rotor equipment, rubber seals, as well as threads of fixed-angle rotors.

other than the one recommended by the centrifuge
manufacturer, please check that the intended method will
not damage the rotors, accessories, or other parts of the
centrifuge. For decontamination, disinfection with alcohol-
containing liquids and autoclaving are the recommended
methods. Below you also find some recommendations for
disinfection reagents based on research done by Eppendorf.

You should not use UV, beta, gamma, or any other high-energy radiation source for disinfection.
Do not use any gassing for disinfection.