Next Advance Frit Kit - Manual (Page 2)

Introduction
The Next >>> Advance Frit Kit provides you with
the reagents and protocol you need to prepare a fused
silica plug. We provide this kit for the purpose of plugging
the end of fused silica capillaries for liquid
chromatography systems. Other applications may utilize
fused silica plugs and may or may not be compatible with
the reagents provided in this kit. Please consult with our
Technical Support prior to using this kit for other
applications as it may not be suitable for your use.
Prior to using the kit, make sure that you have all the
reagents listed in the table below and the
Next >>>
Advance pressure cell for loading your
chromatography stationary phase after frit preparation.
Included in this kit
Item Comments
0.5mL formamide green capped tube
molecular biology grade
Formamide activates the silicate
solution to form the frit
1.5mL Kasil ® 1624 white white capped tube
1.0mL Kasil ® 1 clear clear capped tube
cutting tool white square for cutting fused
silica LC column tubing
instruction booklet this document (hello!)
NOT Included in this kit
Item Comments
pressure cell to apply pressurized gas for
compression of stationary phase
into the capillary tube
stationary phase C4, C8, C18, etc.
glassware small beaker, glass vial
buffers and solvents
Revision 2009.01.11 © 2008 Next Advance
Instructions
1. Into a small glass vial (1 or 2 dram), pipet 300!L
Kasil ® 1624 (potassium silicate). OPTIONAL: Add
100!L Kasil ® 1 and mix with the Kasil ® 1624.
This step has been reported to improve the
fidelity and strength of frits.
2. Add 100ul formamide. Mix well via pipet.
3. Quickly dip the end of the fused silica tubing into the
mixture in the vial. The surface tension of the liquid
will draw it up into the capillary. NOTE: Dipping the
end for too long will result in large silica plugs and
larger dead volumes in the column.
4. To cure the solution into a porous silica plug, place
the tubing into an oven (at 100 °C) for four hours or
overnight.
5. The resulting plugs/frits can be stored in a dry clean
area until you are ready to use them.
6. Trim the tubing after curing the frit. This will remove
excess glass frit material so as to reduce the dead
volume of the column.
7. Now your column is ready to be loaded with your
stationary phase.
References
Maiolica, A.; Borsotti, D. & Rappsilber, J. “Self-made frits
for nanoscale columns in proteomics” PROTEOMICS,
2005, 5, 3847-3850.
Making a Kasil Frit – MS Wiki
http://www.proteomicswiki.org/wiki/index.php/Making_Kasil_Fri
t
Revision 2009.01.11 © 2008 Next Advance