GE ELECTROPHORESIS EPS 301 - Manual (Page 8)

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View actual values
Press VIEW in Run mode to change between the actual values for voltage, current or elapsed time. Press
VIEW for more than three seconds to switch automatically between these values. Stop the automatic
switching by pressing VIEW or RUN.
View programmed values
Press SET/ENTER to view the programmed values during a run. If the keyboard is idle for 4 seconds or
you press RUN the display goes back to the actual values.
Change parameters during a run
When the method is running, changes can be introduced in the program by pressing SET/ENTER and
using / . Confirm by pressing SET/ENTER or RUN
Stop the run
When the programmed time has elapsed, the program will enter the End mode and the alarm will sound.
You can stop the alarm by pressing any key. It is also possible to break the run manually by pressing
STOP. In both cases, the values for voltage and current will go to zero, as indicated by the light emitting
diode switching off.
View end parameter values
Display the end parameter values by pressing VIEW.
Post electrophoresis
Disconnect the leads and proceed with post-electrophoretic techniques. Since the quality of your
separation will deteriorate due to diffusion, you should remove the gel and begin staining or blotting
immediately.
5.4 Short instructions
This section summarises the main points covered earlier in this chapter. Use it as a check list once you
are familiar with the detailed programming and running instructions. Refer also to the separate short
instruction EPS 301 included with the power supply. We recommend you keep this separate short
instruction close by the instrument.
1. Turn mains power ON. The program enters the End mode. If the programmed values from the last
run are to be used again, go to point 4 below. To change the program, press SET/ENTER to enter the
program mode. The voltage symbol V is lit and the value for voltage flashes. The programmed value
from the last run is displayed. Use /
to set to the desired voltage. Press SET/ENTER to confirm.
If the voltage value is correct from the beginning just press SET/ENTER to confirm.
2. The display shows the last programmed value for current flashing. Press / until the desired value
is reached. Confirm by pressing SET/ENTER.
3. The value for time is now flashing. Use / to set the time. To switch off the timer, set the time to
zero. Indicated by - - - -. Confirm by pressing SET/ENTER.
4. When programming is completed, connect your electrophoresis unit to the output(s), and press RUN.
5. Press VIEW to display actual voltage, current and elapsed time. Press VIEW for more than three
seconds to start automatic switching of actual values.
6. Press SET/ENTER during the run to check the programmed parameters and change the values by
pressing / . Confirm by SET/ENTER.
7. The program stops automatically on completion or on STOP being pressed.
8. Press VIEW after the run to check the end parameters.
EPS 301 - User Manual