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Bio-Rad Ligation and Transformation Module User Manual

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Optional Accessories
Vortex mixer (catalog #166-0610EDU)

Vacuum source

Agarose electrophoresis equipment

GAPDH PCR module (catalog #166-5010EDU)

PCR Kleen™ Spin Purification module (catalog #732-6300EDU)

pGLO™ Plasmid, 20 µg (catalog #166-0405EDU)

Aurum™ Plasmid Mini Purification Module (catalog #732-6400EDU)

Electrophoresis reagents:

Small Ethidium Bromide DNA Electrophoresis Reagent Pack (catalog
#166-0451EDU)

Small Fast Blast™ DNA Electrophoresis Reagent Pack (catalog
#166-0450EDU)

Sample Loading Dye, 5x, 1 ml (catalog #166-0401EDU)

EZ Load™ 500 bp Molecular Ruler (catalog #170-8354EDU)

Sequencing and Bioinformatics module (catalog #166-5025EDU)

Refills Available Separately

Ligation module reagent refill (catalog #166-5016EDU)

Includes T4 DNA ligase, 2x ligation reaction buffer, proofreading
polymerase, pJet1.2 blunted vector, sterile water

Bgl II reagent refill (catalog #166-5018EDU)

Includes Bgl II restriction enzyme and 10x Bgl II reaction buffer

Transformation module reagent refill (catalog #166-5017EDU)

Includes transformation reagent A, transformation reagent B, 1 M IPTG,
C-growth medium

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