Bio-Rad Quick Start™ Bradford Protein Assay - Manual (Page 33)

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Microplate Standard Assay
Standard Source of Diluent Final
Tube # Volume (µl) Standard Volume (µl) [Protein] (µg/ml)
1 20 2 mg/ml stock 0 2,000
2 30 2 mg/ml stock 10 1,500
3 20 2 mg/ml stock 20 1,000
4 20 Tube 2 20 750
5 20 Tube 3 20 500
6 20 Tube 5 20 250
7 20 Tube 6 20 125
8 (blank) – – 20 0
2 ml Microassay Cuvette Standard Dilutions
Standard Source of Diluent Final
Tube # Volume (µl) Standard Volume (µl) [Protein] (µg/ml)
1 40 2 mg/ml stock 3,160 25
2 65 2 mg/ml stock 6,435 20
3 30 2 mg/ml stock 3,970 15
4 3,250 Tube 2 3,250 10
5 3,250 Tube 4 3,250 5
6 3,250 Tube 5 3,250 2.5
7 3,000 Tube 6 3,000 1.25
8 (blank) – – 3,200 0
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