Table 3. buffer formulations – Bio-Rad Bio-Scale™ Mini Affi-Gel® Blue Cartridges User Manual

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Table 3. Buffer formulations.

Application Buffers

A. 0.028 M NaCl, 0.020 M Tris-HCl, pH 8.0

B. 0.020 M K

2

HPO

4

, pH 8.0

C. 0.020 Na

2

HPO

4

, pH 7.1

Elution Buffers

D. 0.4 M K

2

HPO

4

, pH 8.0

E. 1.4 M NaCl, 0.020 M Tris-HCl, pH 8.0

F. 1.4 M NaCl, 0.020 M Na

2

HPO

4

, pH 7.1

Regeneration Buffers

G. 1.4 M NaCl, 0.10 M acetic acid, pH 3.0, 40% v/v isopropyl

alcohol

H. 1.5 M sodium thiocyanate in application buffer A, B, or C

I.

2.0 M guanidine-HCl in application buffer A, B, or C

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