Bio-Rad Bio-Plex Pro™ Human Chemokine Assays - Manual (Page 26)

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Note: Do not use preset panels found in Bio-Plex Manager software
version 5.0 or earlier, as the bead regions are not up to date.
3. Click Format Plate and format the plate according to the plate layout
created in section 1 (Plan Plate Layout). To modify the plate layout,
follow the steps below (see Figure 4).
a. Select the Plate Formatting tab.
b. Select the standards icon
S
and drag the cursor over all the
wells that contain standards. Repeat this process for
Blanks B , Controls
C
, and Samples
X
. Note that
Bio-Plex Manager automatically subtracts the blank MFI
value from all other assay wells.
4. Click Enter Standards Info in the Protocol Settings bar.
a. Enter the highest concentration of each analyte in the top row
(labeled S1) of the table. S1 concentration information is included
with each vial of standards.
Fig. 4. Plate formatting.