17 connecting the separation column – Metrohm 930 Compact IC Flex ChS/PP User Manual
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3.17 Connecting the separation column
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930 Compact IC Flex ChS/PP (2.930.1300)
3 Connecting the guard column
CAUTION
When inserting the guard column, ensure that it is inserted cor-
rectly based on the marked flow direction (if specified).
￭
Fasten the input of the guard column to the column input capil-
lary using a short pressure screw (6.2744.070).
￭
Optional: In case the guard column is connected to the separation
column using a provided a connection capillary, fasten the con-
nection capillary to the guard column's output using the pressure
screw also provided.
Rinsing the guard column
1 Rinsing the guard column
￭
Place a beaker under the guard column's output.
￭
Start manual control in MagIC Net and select the high-pressure
pump: Manual
▶ Manual control ▶ Pump
– Flow: in accordance with column leaflet
– On
￭
Rinse the guard column with eluent for approx. 5 minutes.
￭
Use manual control in MagIC Net to stop the high-pressure pump
again: Off.
3.17
Connecting the separation column
The intelligent separation column (iColumn) is the heart of the ion chro-
matographic analysis. It separates different components according to their
interactions with the column. Metrohm separation columns are equipped
with a chip where their technical specifications and history (start-up, oper-
ating hours, etc) are stored.
NOTE
Information regarding which separation column is suitable for your
application can be found in the Metrohm IC Column Program, the
(Ion Chromatography product area) or obtained
directly from your representative.